Product Description:
Advantages:
Unbiased representation of 5’ and 3’ mRNA transcript ends
Sensitive detection of low copy number transcripts
High cDNA yields from as little as 1pg total RNA
Simple 2-tube system
Wisent cDNA Synthesis Kit uses the latest technology in reverse transcription buffer chemistry to enhance cDNA synthesis speed and yield, with accurate representation of transcript ends. The reverse transcriptase (RTase) is thermostable and highly active, and is blended with an RNase inhibitor. The RTase is not inhibited by ribosomal RNA (rRNA) or transfer RNA (tRNA), and total RNA is an ideal substrate. The 5× cDNA synthesis mix can be used with up to 4.0μg total RNA. The relative concentrations of random hexamers and anchored oligo(dT) have been optimized for generating cDNA suitable for real-time PCR experiments.
This mix is optimized for generating cDNA for downstream real-time PCR analysis.
Ordering Information:
CAT.NO. | Quantity | Specification |
801-100-XR | 25 REACTIONS | cDNA synthesis |
801-100-AR | 100 REACTIONS | cDNA synthesis |
Product Stability And Storage:
On arrival store at -25°C to -15℃.
Avoid prolonged exposure to light.
If stored correctly the kit will retain full activity for 12 months. It is stable at 2-8°C for one month and can withstand 30 freeze/thaw cycles without any loss of activity.
