HEPES 缓冲液，330-050

Product Description:
WISENT HEPES buffer is a 1 M solution prepared with high-purity HEPES (free acid), high-quality NaOH and cell culture-grade ultrapure water, and sterilized by filtration. 1 M HEPES buffer (4-hydroxyethylpiperazine ethanesulfonic acid), after sterile filtration, is an essential biological buffer for cell biology, biochemistry and general biological research. HEPES is an amphoteric ionic buffer effective within the pH range of 6.8 to 8.2 and suitable for a wide range of research applications. HEPES can serve as a buffer for cell culture media and offers multiple advantages over Tris and phosphate buffers. In particular, it exhibits lower cytotoxicity and maintains optimal cell condition and viability throughout experiments.
In addition, it can maintain the physiological pH of cells, protect cells from pH fluctuations caused by respiratory carbon dioxide, and maintain cellular homeostasis. This property is superior to that of bicarbonate buffer (NaHCO₃). Beyond its excellent performance in cell culture, HEPES can also be applied in techniques such as immunoprecipitation, cell lysis, and live‑cell imaging, providing a stable and well‑controlled experimental environment. Featuring versatile functions, strong pH buffering capacity and low interaction with other biomolecules, HEPES is an indispensable reagent across many research fields.
The routine working concentration of HEPES in cell culture ranges from 5 mM to 30 mM. However, concentrations exceeding 40 mM may be cytotoxic to certain cell types and inhibit cell growth. HEPES is widely used in various applications including tissue culture. It is commonly used to stabilize the pH of culture media incubated in contact with ambient air.