Product Description:Hepato MAX is a cell culture medium system that supports the growth and expansion of hepatocyte. The Wisent Hepato MAX Cell Culture System is supplied as a set consisting of sterile liquid basal culture medium (Hepato MAX Medium, CAT. NO.: 301-070-CL) and a frozen supplement (Hepato MAX Supplement, CAT. NO.: 301-071-XL) containing hormones and growth factors.Fetal Bovine Serum (FBS) must be added to the medium at the recommended concentration to support cell growth. We recommend using Australian-origin Premium Grade FBS (CAT. NO.: 085-150).
CAT.NO. | DESCRIPTION | SIZE |
301-070-CL | Serum free medium and supplement for liver cells | 500 mL |
301-071-XL | 10 mL |
Instrutions For Use:
Preparation of the Medium for Use:
Complete medium is obtained by addition of the Hepato MAX Supplement (CAT.NO.: 301-071-XL), Premium FBS (CAT.NO.: 085150) and Antibiotics in the Hepato MAX Basal Medium (CAT.NO.: 301-070-CL).
1. Thaw Hepato MAX Supplement between 15℃ and 25℃.
2. Remove the safety seal and carefully open the basal medium in a laminar flow cabinet.
3. Remove the safety seal and carefully open screwtop of the supplement and transfer the contents with a sterile pipette to the medium. Repeat with FBS and Antibiotics.
4. Note the date of addition of the supplement on the bottles.
5. Close the bottle and swirl gently until a homogenous mixture is formed. The complete medium should be stored in the dark between 2 to 8℃ and can be kept for 6 weeks.
Storage conditions:
The Hepato Max Medium should be stored in the dark at 2 to 8℃ for up to 18 months. DO NOT freeze the medium as it could cause irreversible damage to the medium. The Hepato Max Supplement has to be stored at -25℃ to -15℃. Do not heat the supplement over 37℃ or use uncontrollable high sources of heat (e.g. microwave appliances.)
