Product Description:WISENT HCELL-300, HCELL-400 and HCELL-500 products are serum-free alternatives for hybridoma cell culture and high-efficiency monoclonal antibody production, containing a very low protein concentration (below 20 mg/L). Traditionally, hybridomas have been cultured in media containing serum. The use of serum in culture presents numerous disadvantages and risks for antibody production. This has driven a trend toward the use of serum-free media for manufacturing purposes. Eliminating the need for serum ensures consistent culture conditions.
CAT.NO. | NAME | DESCRIPTION | SIZE |
001-055-CL | HCELL-300 | Serum free media for hybridoma cells 杂交瘤细胞无血清培养基 | 500 mL |
001-065-CL | HCELL-400 | 500 mL | |
001-075-CL | HCELL-500 | 500 mL |
Instructions For Use:
Adaptation Of Cells To HCELL Serum-free Media :
Adaptation will depend on the particular cell line. For some cell lines direct adaptation is possible while others require sequential adaptation. In both cases cells must be used in mid-logarithmic growth phase with high viability.
1) Direct Adaptation
1.Transfer hybridoma cells growing in media containing serum to HCELL medium which has been prewarmed to 37°C. Seeding density should be double the normal seeding density for the particular cell line.
2. Grow cells until viable cell density reaches 1 × 106/mL. Subculture the cells to a cell density of 1 × 105/mL in fresh HCELL medium for 3 passages.
3. If the culture fails to maintain viability over 3 passages, use the sequential adaptation method.
2) Sequential Adaptation
1. Inoculate hybridoma cells at twice the standard seeding density in a 75:25 (v/v) mixture of serum-containing medium and HCELL.
2. As soon as the culture density reaches 1 × 106 viable cells/mL subculture into a 50:50 (v/v) mixture of serum containing media and HCELL.
3. As soon as the culture density reaches 1 × 106 viable cells/mL subculture into a 25:75 (v/v) mixture of serum containing media and HCELL.
4. As soon as the culture density reaches 1 × 106 viable cells/mL subculture into a 100% HCELL medium.
FORM: Liquid, Sterile, Ready to use.
SHEIF LIFE: Maximum 18 months.
STORAGE TEMPERATURE: STORE AT 2 TO 8℃. Protect from light.
