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Hepato MAX, 肝细胞无血清培养基及添加剂, 301-070

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CAT.NO.

DESCRIPTION

SIZE

301-070-CL

Serum free media and supplement for liver cells
肝细胞无血清培养基及添加剂

500 mL

301-071-XL

10 mL

Product Description:

Hepato MAX is a cell culture media system that supports the growth and the expansion of Liver cells. Wisent Hepato MAX Cell Culture System is supplied in a format that contains a sterile liquid basal culture medium (Hepato Max Medium cat no: 301-070-CL), and a frozen supplement (Hepato MAX cat no: 301-071-XL), which contains hormones and growth factors. FBS (we suggest Premium Grade FBS cat no: 085150) needs to be added according to the percentage required for cell growth.

 

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Preparation of the Medium for Use

Complete medium is obtained by addition of the Hepato MAX Supplement (cat no: 301-071-XL), Premium FBS (cat no: 085150) and Antibiotics in the Hepato MAX Basal Medium (cat no: 301-070-CL).

1. Thaw Hepato MAX Supplement between 15℃ and 25℃.

2. Remove the safety seal and carefully open the basal medium in a laminar flow cabinet.

3. Remove the safety seal and carefully open screwtop of the supplement and transfer the contents with a sterile pipette to the medium. Repeat with FBS and Antibiotics.

4. Note the date of addition of the supplement on the bottles.

5. Close the bottle and swirl gently until a homogenous mixture is formed. The complete medium should be stored in the dark between 2 to 8℃ and can be kept for 6 weeks.

Stability and Storage

The Hepato Max Medium should be stored in the dark at 2 to 8ºC for up to 18 months. DO NOT freeze the medium. This can lead to high salt concentrations by freezing out pure water and would cause irreversible damage. The Hepato Max Supplement has to be store at -25ºC to -15℃. Do not heat the supplement over 37ºC or use uncontrollable high sources of heat (e.g. microwave appliances.)

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