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ADVANCEDtm qPCR mastermix with SUPERGREENtm dye

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ADVANCED™Supergreen™qPCR Mastermix
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介绍详情

Ordering Information: 

CAT.NO.

Quantity

Specification

800-431-UL

1 x 1 mL

LOW ROX

800-433-UL

3 x 1 mL

LOW ROX

800-435-UL

5 x 1 mL

LOW ROX

800-441-UL

1 x 1 mL

HIGH ROX

800-443-UL

3 x 1 mL

HIGH ROX

800-445-UL

5 x 1 mL

HIGH ROX

Also available in 50 mL bulk format,please inquire.

For instrument compatibility refer to APPENDIX I

Product Description:

WISENT ADVANCED mastermix takes advantage of the latest advancements in polymerase technology and uses advanced buffer technology offering market leading performance. The Supergreentm dye does not inhibit PCR, unlike other dyes. The mastermix uses a unique small molecular inhibitor technology that prevents formation of primer dimers. The buffer system allows efficient amplification from GC-rich and AT-rich templates. The product is intended for in vitro research purposes.

 

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Product Stability And Storage:

On arrival store at -25°C to -15℃.

Avoid prolonged exposure to light.

If stored correctly the mastermix will retain full activity for 12 months. It is stable at 2-8°C for 1 month and can go through 30 freeze/thaw cycles with no loss of activity.

Instructions For Use:

Note: For efficient amplification under fast cycling conditions we recommend amplicon lengths between 80bp and 200bp. Do not exceed 400 bp. The shorter the amplicon length the faster the reaction can be cycled. Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings.

Reaction Setup:

1 Before starting, briefly vortex 2x WISENT ADVANCED MASTERMIX .

2 Prepare a final mix , recommended 20 μL, as follows:

Add

Volume

Final concentration

WISENT ADVANCED MASTERMIX

10 μL

1X

Forward primer (10 μM)

0.8 μL

400 nM

Reverse primer (10 μM)

0.8 μL

400 nM

Template

cDNA  :< 100 ng
genomic DNA : < 1 μg

variable

Add PCR grade water up to 20 μL final volume

3 Program your instrument using following conditions, acquiring data on the SYBR® Green or FAM channel: 

Cycles

Temperature

Time

Notes

1

95°C

2min: cDNA
3min: genomic DNA

Polymerase activation

40

95°C

5 seconds

Denaturation

 

 

60°C to 65°C

 

20-30 seconds

Anneal/Extension, do not exceed 30 seconds, do not use temperatures below 60°C

Melt analysis

Refer to instrument instructions

optional

APPENDIX I

HIGH ROX / LOW ROX INSTRUMENT COMPATIBILITY

FOR FOLLOWING KITS:

-WISENT ADVANCEDtm qPCR MASTERMIX WITH SUPERGREENtm DYE

-WISENT ADVANCEDtm qPCR 1 step mastermix with SUPERGREENtm dye

-WISENT ADVANCEDtm qPCR PROBE 1 step mastermix

-WISENT ADVANCEDtm qPCR GENOTYPING mastermix

Manufacturer

Instrument

Lo-ROX

Hi ROX

Analytica Jena

qTower

Yes

Yes

Applied Biosystems

7500, 7500 FAST, Viia7TM

Yes

No

Applied Biosystems

7000, 7300,7700,7900, 7900HT, 7900HT FAST, StepOneTM, StepOneTM Plus

No

Yes

Bio-Rad®

iCycler®, MyiQ®, iQ TM5, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM

Yes

No

Cepheid®

Smartcycler®

Yes

Yes

Eppendorf

Mastercycler® ep realplex, Mastercycler® realplex 2S

Yes

Yes

Illumina®

EcoTM

Yes

Yes

Qiagen/Corbett

Rotor-GeneTM 3000, 6000, Q

Yes

Yes

Roche Applied Science

Lightcycler®480, Lightcycler®Nano

Yes

Yes

Stratagene (Agilent)

MX 4000P®, MX 3000P®, MX 3005P®

Yes

No

Takara

Cycler Dice®

Yes

Yes

Techne

Quantica®

Yes

Yes

Note: when offered No-ROX is compatible with all machines above. 

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