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ADVANCEDtm qPCR PROBE mastermix

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浏览量:
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所属分类
ADVANCEN™qPCR PROBE Mastermix
数量
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1
介绍详情

CAT.NO.

DESCRIPTION

REMARKS 

SIZE

801-071-AR

Compatible on all real-time PCR platforms and various
template(genomicDNA,cDNA)high efficiency in multiplex
reactions,increased limit of detections
适用于所有的探针法(如TAQMAN )定量PCR，
多重反应的高效扩增，
适用于各种DNA模板(基因组DNA , CDNA )
灵敏度高，可以高效检测低拷贝目的序列

 

 

 

 

 

 

 

 

LOW ROX

 

 

100 reactions

801-071-ER

500 reactions

801-071-QR

2000 reactions

801-072-AR

HIGH ROX

 

 

100 reactions

801-072-ER

500 reactions

801-072-QR

2000 reactions

801-073-AR

NO ROX

 

 

100 reactions

801-073-ER

500 reactions

801-073-QR

2000 reactions

Product Description:

WISENT ADVANCED PROBEmastermix has following advantagers:

· High efficiency in multiplex reactions

· Rapid extension rate for early Ct values

· Market leading sensitivity - increased limit of detection

· Compatible on all real-time PCR platforms - standard and fast cycling conditions

Wisent Probe Mix is a universal probe kit designed for use in all probe based real-time PCR assays. Whether your application is for a singleplex or multiplex expression study, a genotyping experiment or a diagnostic assay,Wisent Probe Mix is the best choice for all your probe based real-time PCR needs.Wisent Probe Mix can be used to quantify DNA templats wether genomic, cDNA and viral sequences. Extremely low copy number targets can be detected with high efficiency. Optimized for use in fast and ultra fast cycling conditions.

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Product Stability And Storage:

On arrival store at -25°C to -15℃.

Avoid prolonged exposure to light.

If stored correctly the mastermix will retain full activity for 12 months. It is stable at 2-8°C for 1 month and can go through 30 freeze/thaw cycles with no loss of activity.

Instructions For Use:

Note: 1 For efficient amplification under fast cycling conditions we recommend amplicon lengths between 80bp and 200bp. Do not exceed 400 bp. The shorter the amplicon length the faster the reaction can be cycled. Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings. Note : 2 For TAQMANR probes choose probe close to 5’ primer and avoid terminal guanosine residues.

Reaction Setup:

1 Before starting, briefly vortex 2x WISENT ADVANCED mix .

2 Prepare a final mix , recommended20 ul, as follows:

Add

Volume

Final concentration

WISENT ADVANCED mix

10 ul

1X

Forward primer (10 uM)

0.8 ul

400 nM

Reverse primer (10 uM)

0.8 ul

400 nM

Probe

0.4ul

200nM

Template DNA

cDNA  :< 100 ng
genomic DNA : < 1 ug

variable

Add PCR grade water up to 20 ul final volume

3 Program your instrument using following conditions, acquiring data on the SYBR® Green or FAM channel: 

Cycles

Temperature

Time

Notes

1

95°C

2min: cDNA
3min: genomic DNA

Polymerase activation

40

95°C

5 seconds

Denaturation

60°C to 65°C

20-30 seconds

Anneal/Extension do not exceed 30 seconds ; do not use bellow 60 °C

FAM channel:

APPENDIX I

HIGH ROX / LOW ROX INSTRUMENT COMPATIBILITY

FOR FOLLOWING KITS:

-WISENT ADVANCEDtm qPCR MASTERMIX WITH SUPERGREENtm DYE

-WISENT ADVANCEDtm qPCR 1 step mastermix with SUPERGREENtm dye

-WISENT ADVANCEDtm qPCR PROBE 1 step mastermix

-WISENT ADVANCEDtm qPCR GENOTYPING mastermix

Manufacturer

Instrument

Lo-ROX

Hi ROX

Analytica Jena

qTower

Yes

Yes

Applied Biosystems

7500, 7500 FAST, Viia7TM

Yes

No

Applied Biosystems

7000, 7300,7700,7900, 7900HT, 7900HT FAST, StepOneTM, StepOneTM Plus

No

Yes

Bio-Rad®

iCycler®, MyiQ®, iQ TM5, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM

Yes

No

Cepheid®

Smartcycler®

Yes

Yes

Eppendorf

Mastercycler® ep realplex, Mastercycler® realplex 2S

Yes

Yes

Illumina®

EcoTM

Yes

Yes

Qiagen/Corbett

Rotor-GeneTM 3000, 6000, Q

Yes

Yes

Roche Applied Science

Lightcycler®480, Lightcycler®Nano

Yes

Yes

Stratagene (Agilent)

MX 4000P®, MX 3000P®, MX 3005P®

Yes

No

Takara

Cycler Dice®

Yes

Yes

Techne

Quantica®

Yes

Yes

Manufacturer

Instrument

Lo-ROX

Hi ROX

Note: when offered No-ROX is compatible with all machines above 

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